'One-pot' CRISPR platform delivers lab-like sensitivity in 30 minutes for at-home testing

A research team from The Hong Kong University of Science and Technology (HKUST) has developed an innovative "one-pot" testing platform, known as TEMPO, that could transform highly sensitive nucleic acid testing from a laboratory-based procedure into a simple, single-step test that can be performed at home. With a single reaction tube, users can obtain results comparable to those of professional laboratory tests in as little as 30 minutes. The breakthrough has the potential to bridge the technological gap between existing rapid tests and laboratory-based nucleic acid diagnostics, offering a more convenient solution for future infectious disease surveillance and genetic screening.

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